Figure 1.
Vimentin-SunTag sparse labeling of endogenous VIFs. (A) Schematic of the vimentin-SunTag system design. (B) Co-expression of the ensemble vimentin reporter vimentin-mCherry (magenta in overlay) and sparse-labeled vimentin-SunTag (yellow in overlay), reused in Fig. S1 D. (C) Inset of the merged image from B (left) with localized SunTag particles overlaid. (D) Micrograph of RPE expressing sparse vimentin-SunTag before (left) or 10 min after (right) permeabilization/extraction with 0.5% Triton X-100. (E) PREM micrographs of a nontransfected RPE (left) or a vimentin-SunTag–expressing RPE (right). 12-nm gold beads affixed to anti-GFP antibodies are pseudocolored yellow. (F) Insets of single VIFs from E with gold-labeled vimentin-SunTag particles. Refer to the image caption for details.

Vimentin-SunTag sparse labeling of endogenous VIFs. (A) Schematic of the vimentin-SunTag system design. (B) Co-expression of the ensemble vimentin reporter vimentin-mCherry (magenta in overlay) and sparse-labeled vimentin-SunTag (yellow in overlay), reused in Fig. S1 D. (C) Inset of the merged image from B (left) with localized SunTag particles overlaid. (D) Micrograph of RPE expressing sparse vimentin-SunTag before (left) or 10 min after (right) permeabilization/extraction with 0.5% Triton X-100. (E) PREM micrographs of a nontransfected RPE (left) or a vimentin-SunTag–expressing RPE (right). 12-nm gold beads affixed to anti-GFP antibodies are pseudocolored yellow. (F) Insets of single VIFs from E with gold-labeled vimentin-SunTag particles.

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