Figure 6.
Local translation of FL RNAs near the PM imaged by TIRFM. (A) Representative images of cells expressing ΔNC, ΔgGag-ΔNC, and gGag-∆NC with or without puromycin or LMB. Yellow arrows point to translation sites. Scale bars are 10 and 2 µm for zoom insets. The dotted line shows the boundaries of the cell and R, G, and M indicate red, green, and merge, respectively. (B) Proportion of translating FL RNA near the PM. The graph shows the mean ± SEM, n ≥ 22 cells, ns = nonsignificant, ****P ≤ 0.0001 (Mann–Whitney test). (C) Effect of LMB on proportion of translating FL RNA of MLV-∆NC. n ≥ 29 cells.

Local translation of FL RNAs near the PM imaged by TIRFM. (A) Representative images of cells expressing ΔNC, ΔgGag-ΔNC, and gGag-∆NC with or without puromycin or LMB. Yellow arrows point to translation sites. Scale bars are 10 and 2 µm for zoom insets. The dotted line shows the boundaries of the cell and R, G, and M indicate red, green, and merge, respectively. (B) Proportion of translating FL RNA near the PM. The graph shows the mean ± SEM, n ≥ 22 cells, ns = nonsignificant, ****P ≤ 0.0001 (Mann–Whitney test). (C) Effect of LMB on proportion of translating FL RNA of MLV-∆NC. n ≥ 29 cells.

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