RNA imaging imaged by TIRFM. (A) Control for TIRF mode with fluorescent beads. NIH3T3 GFP/RFP cells were transfected with ΔNC and fixed. Red channel is shown in gray. White arrows indicate the fluorescent beads (100 nm) deposited on the coverslip a few minutes before acquisition. Epifluorescence (left) and TIRF (right) were captured at the same focal plane. Raw images are presented, and scale bar is 10 µm. (B) Representative images of FL and NV RNAs at the periphery of GFP/RFP cells expressing MLV-ΔNC or NV-MS2. Scale bars are 10 µm, and the dotted line indicates the boundaries of the cell.