Arl8b is on vesicles recruited by mito-VPS41. (A) Original EM images of Fig. 6 without colored organelles. (B) Original EM images of Fig. 7 without colored organelles. (C) Original EM images of Fig. 8 without colored organelles. (D) Original EM images of Fig. 9 without colored organelles. (E) Confocal images showing the CI-M6PR distribution in HeLa^VPS41KO cells expressing Arl8b-HA together with either mito-VPS41^WT-GFP or negative control mito-GFP. Scale bars: 10 µm. (F) Fluorescence image showing the LysoTracker dye distribution after 30 min of incubation in HeLa^VPS41KO cells expressing Arl8b-HA together with mito-VPS41^WT-GFP (top) or mito-GFP (middle). The bottom line shows staining for Arl8b-HA in cells used for LysoTracker detection. Scale bars: 10 and 5 µm (inset). (G) Confocal images of PC12^VPS41KO cells expressing mito-VPS41^WT-GFP and Arl8b-mCherry and immunolabeled for endogenous LAMP1. Scale bars: 5 µm. (H) Confocal images showing the endogenous localization of AP3D in PC12^VPS41KO cells transfected with Arl8b-mCherry and either mito-GFP or mito-VPS41^WT-GFP. Scale bars: 5 µm.