Figure 7.
LAMP1/Arl8b-positive vesicles accumulate around mitochondria in cells expressing mito-VPS41 HeLaVPS41KOcells transfected with mito-VPS41-V5 and Arl8b-GFP and immunolabeled for GFP and endogenous LAMP1. (A) LAMP1 (10-nm gold) colocalizes with Arl8b (15-nm gold) on small vesicles (blue) in the TGN area and around mitochondria (M; purple). (B) Higher magnification of LAMP1/Arl8b-positive vesicles (blue) attached to mitochondria. (C) In addition to LAMP1-positive vesicles (blue), a cytosolic pool of Arl8b is present on mitochondria (M; purple). LAMP1 and Arl8b also colocalize on endosomes (E). E, endosome (yellow); G, Golgi complex; M, mitochondria (purple); vesicles (blue); TGN, trans-Golgi network; P, plasma membrane. Scale bars: 200 nm. For original EM images, see Fig. S3 B.

LAMP1/Arl8b-positive vesicles accumulate around mitochondria in cells expressing mito-VPS41 HeLa VPS41KO cells transfected with mito-VPS41-V5 and Arl8b-GFP and immunolabeled for GFP and endogenous LAMP1. (A) LAMP1 (10-nm gold) colocalizes with Arl8b (15-nm gold) on small vesicles (blue) in the TGN area and around mitochondria (M; purple). (B) Higher magnification of LAMP1/Arl8b-positive vesicles (blue) attached to mitochondria. (C) In addition to LAMP1-positive vesicles (blue), a cytosolic pool of Arl8b is present on mitochondria (M; purple). LAMP1 and Arl8b also colocalize on endosomes (E). E, endosome (yellow); G, Golgi complex; M, mitochondria (purple); vesicles (blue); TGN, trans-Golgi network; P, plasma membrane. Scale bars: 200 nm. For original EM images, see Fig. S3 B.

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