Mito-VPS41 recruits the GTPase Arl8b. (A) Confocal images of HeLa^VPS41KO cells cotransfected with mito-VPS41^WT-V5 and GFP-Rab7, Arl8b-GFP, Rab2-GFP, Rab6-GFP, or GFP empty vector and labeled for V5. Dashed boxes are enlarged in the next images. (B) Quantification of mitochondrial recruitment of indicated GTPases upon the expression of mito-VPS41^WT-V5. Values are the mean ± SEM. n > 40. Statistical significance was determined using Tukey’s multiple comparisons test. ****P < 0.0001, ns: not significant. (C) Confocal images of HeLa^VPS41KO, HeLa^VPS18KO, and HeLa^VPS39KO cells co-expressing Arl8b-GFP and mito-V5, mito-VPS41^R662*-V5, or mito-VPS41^WT-V5 and labeled for V5. (D) Quantification of mitochondrial recruitment of Arl8b-GFP by indicated constructs in C. Statistical significance was determined using Tukey’s multiple comparisons test. ****P < 0.0001, ***P < 0.001, ns: not significant, mean ± SEM. n > 25. Scale bars: 20 and 5 µm (inset magnification).