Analyses of hair follicle stem cell organoids from CerS4-deficient mice. (A and B) FACS-based quantification of live cells (A) and CD34⁻ integrin α6⁺ non-HFSCs (B) from Ctr and CerS4^epi−/− organoids cultured for indicated times (n = 4 mice/genotype for 14 days, six mice/genotype for all other time points; mean ± SD; unpaired t test). (C) FACS-based quantification of annexin-positive cells of Ctr and CerS4^epi−/− organoids cultured for indicated time points (n = 6 mice/genotype; mean ± SD; unpaired t test). (D and E) FACS-based quantification of EdU-positive live cells in CD34⁺ integrin α6⁺ HFSCs (D) and CD34⁻ integrin α6⁺ non-HFSCs (E) after a 24-h chase in organoids cultured for indicated time points (n = 6 mice/genotype; mean ± SD; unpaired t test). (F) GO-term enrichment analysis from proteins downregulated in CerS4^epi−/− organoids. (G) GSEA of known markers of distinct skin hair follicle progenitor cell linages (Joost et al., 2016) from differentially expressed proteins in Ctr and CerS4^epi−/− organoids. NES, normalized enrichment score.