Junctional myosin in interfaces is dependent on angle. (A) Heat map of the rate of change in interface length (red: contraction; blue: elongation) as a function of time and interface orientation angle. (n = 1,210 interfaces, k = 3 embryos). (A′) Heat map of the rotation rate (orange: clockwise, purple: counterclockwise) as a function of time and interface orientation angle, for same data set as A. (A″) Heat map of normalized myosin intensity (red: positive intensity; blue: negative intensity) as a function of time and interface orientation angle, for same data set as A. (B) Time evolution of the interface myosin intensity and interface angle in a sample interface. The color of the markers indicates time (going from blue to red). (B′) Interface myosin intensity and angle as a function of time for the same sample interface. (C) Raw microscope images of the same sample interface at 4, 16 and 22 min into germband extension in E-cadherin and Myosin channels. (Scale bars = 5 µm) An additional interface example is given in Fig. S5, C, C′, and D with the same graphs and images as in Fig. 5, B, B′, and C. (D) Orientation angle and myosin intensity evolution in transverse interfaces. (n = 87 interfaces, k = 3 embryos) See Fig. S5 B for boxplot of the distribution of values in grey shaded areas.