Effects of Rho kinase inhibition and Torso disruption. (A) Heatmaps of the rate of change in interface length (red: contraction, blue: elongation) as a function of time and interface orientation angle for movies of small-molecule-treated embryos, specifically 25 mM Y27632 (Rho kinase inhibition, disrupts all pools of myosin) (n = 1,477 interfaces, k = 3 embryos), as well as genetic disruptions, including KOs of Torso (disrupts posterior midgut formation) (n = 2,665 interfaces, k = 5 embryos). The appropriate control for Y27 injection is “H2O injected,” while the appropriate control for Torso KO is “WT.” (B) Heat map of the rotation rate (orange: clockwise, purple: counterclockwise) as a function of time and interface angle for the same data sets as in A. (C) Probability distributions of contraction/elongation rates of the treatments in Figs. 4 and S4. (D) Probability distribution of rotation rates of the treatments in Figs. 4 and S4.
