Figure 4.
Extensive interactions between R. parkeri and the RER network. (A) FIB-SEM image stacks and 3D rendering of ER–rickettsia structures in A549 cells infected with the sca2::Tn mutant (magenta) at 30 hpi. The top image shows a subvolume of the FIB-SEM image stack acquired with the entire ER network (cyan) and the sca2::Tn mutant displayed. The bottom image only shows 3D rendering of the ER membranes adjacent to the bacterial membranes. Scale bar, 1 µm. (B) FIB-SEM slice of a sca2::Tn mutant bacterium (magenta) from A. Insets at right show electron-dense regions (arrow) connecting the rickettsial (magenta) and ER (cyan) membranes. Scale bar, 100 nm. Refer to the image caption for details.

Extensive interactions between R. parkeri and the RER network. (A) FIB-SEM image stacks and 3D rendering of ER–rickettsia structures in A549 cells infected with the sca2::Tn mutant (magenta) at 30 hpi. The top image shows a subvolume of the FIB-SEM image stack acquired with the entire ER network (cyan) and the sca2::Tn mutant displayed. The bottom image only shows 3D rendering of the ER membranes adjacent to the bacterial membranes. Scale bar, 1 µm. (B) FIB-SEM slice of a sca2::Tn mutant bacterium (magenta) from A. Insets at right show electron-dense regions (arrow) connecting the rickettsial (magenta) and ER (cyan) membranes. Scale bar, 100 nm.

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