Figure 3.
R. parkeri is partially wrapped by the rough ER in different cell types. (A and B) TEM images at 30 hpi of A549 (A) and HMEC-1 (B) cells infected with sca2::Tn mutant bacteria. R, rickettsia (arrowhead), RER-wrapped bacterium (arrow), mitochondria–RER interaction. Insets—bacteria (magenta) and ER (cyan). Scale bar, 1 µm; inset, 500 nm. (C) 2D morphometric analysis examples are used to quantify the average bacterial perimeter covered by the RER (left) or the average distance between RER and bacterial membranes (right) (from cells in A). ER, cyan; yellow lines represent traces measured. (D and E) Percentage of the bacterial or mitochondrial perimeter covered by the RER and the average distance between the membranes. Each dot represents an individual bacterium or mitochondrion (>10 counted). Each dot represents an individual bacterium or mitochondrion. Data shown are mean ± SD from an independent replicate (unpaired two-tailed t test). Refer to the image caption for details.

R. parkeri is partially wrapped by the rough ER in different cell types. (A and B) TEM images at 30 hpi of A549 (A) and HMEC-1 (B) cells infected with sca2::Tn mutant bacteria. R, rickettsia (arrowhead), RER-wrapped bacterium (arrow), mitochondria–RER interaction. Insets—bacteria (magenta) and ER (cyan). Scale bar, 1 µm; inset, 500 nm. (C) 2D morphometric analysis examples are used to quantify the average bacterial perimeter covered by the RER (left) or the average distance between RER and bacterial membranes (right) (from cells in A). ER, cyan; yellow lines represent traces measured. (D and E) Percentage of the bacterial or mitochondrial perimeter covered by the RER and the average distance between the membranes. Each dot represents an individual bacterium or mitochondrion (>10 counted). Each dot represents an individual bacterium or mitochondrion. Data shown are mean ± SD from an independent replicate (unpaired two-tailed t test).

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