Figure 2.
The sca2::Tn mutant displays increased interactions with the ER. (A) Percentage of RpBFP or sca2::Tn mutant bacteria forming vacuole-like ER structures at 48 hpi. RpBFP data are independent of that shown in Fig. 1. Each dot represents the mean percentage of bacteria interacting with the ER for three independent experiments (>4,000 bacteria per independent experiment). Error bars represent SEM (unpaired two-tailed t test). (B) Relative frequency of RpBFP or sca2::Tn mutant bacteria forming protrusive or vacuole-like ER structures at 48 hpi. Error bars indicate SEM from three independent experiments (>100 bacteria counted per independent experiment). (C) Live-cell imaging snapshots at 48 hpi of sca2::Tn mutant bacteria (magenta) infecting A549-mCherry-Sec61β cells (cyan). Scale bar, 5 µm; inset, 1 µm. (D) Live-cell imaging snapshots as in C showing cells with (present) or without (absent) vacuole-like ER structures at 48 hpi. Scale bar, 5 µm; inset, 1 µm. (E) Number of bacteria in cells at 48 hpi with vacuole-like ER structures present or absent (images in D). The mean ± SEM of three independent experiments (large outlined circles) are superimposed over the raw data from individual cells (small unlined circles) (>10 cells counted per independent experiment, data are colored by experiment). Refer to the image caption for details.

The sca2::Tn mutant displays increased interactions with the ER. (A) Percentage of RpBFP or sca2::Tn mutant bacteria forming vacuole-like ER structures at 48 hpi. RpBFP data are independent of that shown in Fig. 1. Each dot represents the mean percentage of bacteria interacting with the ER for three independent experiments (>4,000 bacteria per independent experiment). Error bars represent SEM (unpaired two-tailed t test). (B) Relative frequency of RpBFP or sca2::Tn mutant bacteria forming protrusive or vacuole-like ER structures at 48 hpi. Error bars indicate SEM from three independent experiments (>100 bacteria counted per independent experiment). (C) Live-cell imaging snapshots at 48 hpi of sca2::Tn mutant bacteria (magenta) infecting A549-mCherry-Sec61β cells (cyan). Scale bar, 5 µm; inset, 1 µm. (D) Live-cell imaging snapshots as in C showing cells with (present) or without (absent) vacuole-like ER structures at 48 hpi. Scale bar, 5 µm; inset, 1 µm. (E) Number of bacteria in cells at 48 hpi with vacuole-like ER structures present or absent (images in D). The mean ± SEM of three independent experiments (large outlined circles) are superimposed over the raw data from individual cells (small unlined circles) (>10 cells counted per independent experiment, data are colored by experiment).

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