Figure S1.
Vacuole-like ER interactions are a unique adaptation by R. parkeri. (A) Live-cell imaging snapshots of A549 cells expressing an ER marker (BiP-mNeonGreen-KDEL, cyan) infected with R. parkeri expressing BFP (magenta) at 28 hpi. The arrowhead in the inset points to R. parkeri in vacuole-like ER structure. Arrows in the inset point to R. parkeri in protrusive structures. Scale bars, 5 µm. (B) Live-cell imaging snapshots at 4 hpi of A549-mCh-Sec61β cells (cyan) infected with DasherGFP-expressing WT or GFP-expressing ΔactA mutant strains of L. monocytogenes (magenta). Scale bar, 5 µm; inset, 1 µm. (C) Live-cell imaging snapshots at 2 hpi of A549-mCh-Sec61β cells (cyan) infected with GFP-expressing WT or icsA::Ω mutant strains of S. flexneri (magenta). Scale bar, 5 µm; inset, 1 µm. Data are representative of three independent experiments. Refer to the image caption for details.

Vacuole-like ER interactions are a unique adaptation by R. parkeri. (A) Live-cell imaging snapshots of A549 cells expressing an ER marker (BiP-mNeonGreen-KDEL, cyan) infected with R. parkeri expressing BFP (magenta) at 28 hpi. The arrowhead in the inset points to R. parkeri in vacuole-like ER structure. Arrows in the inset point to R. parkeri in protrusive structures. Scale bars, 5 µm. (B) Live-cell imaging snapshots at 4 hpi of A549-mCh-Sec61β cells (cyan) infected with DasherGFP-expressing WT or GFP-expressing ΔactA mutant strains of L. monocytogenes (magenta). Scale bar, 5 µm; inset, 1 µm. (C) Live-cell imaging snapshots at 2 hpi of A549-mCh-Sec61β cells (cyan) infected with GFP-expressing WT or icsA::Ω mutant strains of S. flexneri (magenta). Scale bar, 5 µm; inset, 1 µm. Data are representative of three independent experiments.

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