Dynamics of CEP97^CP110 and CPAP on slowly growing MT plus ends. (A) Upper panel: Kymographs representing bleaching of CPAP-N_WT-mCh (magenta) with a 561-nm laser and its quick recovery. Inset shows the bleaching moment with a white arrow. Bottom panel: Kymographs showing bleaching of both CEP97^CP110-GFP (green) and CPAP-N_WT-mCh (magenta) with a 488-nm laser (white arrow) illustrating that CEP97^CP110-GFP does not recover and CPAP-N_WT-mCh recovers slowly. (B) Upper plot: Mean + SD of the normalized intensity of CEP97^CP110-GFP (green line, n = 30) and CPAP-N_WT-mCh (magenta line, n = 36) over time on slowly growing MT plus ends. Bottom plot: Comparison of the recovery CPAP-N_WT-mCh alone (n = 8) and in the presence of CEP97^CP110-GFP (the latter data are the same as in the lot above). Black arrow marks the time point of photobleaching. (C) Scheme of flow-in assays. (D) Top: Kymographs representing a MT that was dynamic before flow in and switched to slow growth after the flow in of CEP97^CP110-GFP (green, white arrowhead) and CPAP-N_WT-mCh (magenta, white open arrowhead). Bottom: Kymographs showing a MT plus end blocked by CEP97^CP110-GFP (white arrowhead) before and after the flow in. The moment of flow in is indicated by a black arrow in both kymographs.