Figure 3.
CEP97^CP110 forms caps at the plus ends of GMPCPP-stabilized MTs. (A) Kymographs showing GMPCPP-stabilized seeds (magenta) in absence (top row) or presence (bottom row) of 3 µM soluble tubulin labelled with TMR-tubulin (gray). (B) Slices through denoised tomograms containing plus ends of MT seeds in the absence or presence of 80 nM CEP97^CP110-GFP in the absence of free tubulin. (C and D) Average PF lengths (C) and average curvatures (D) of PFs with nonzero length for GMPCPP seeds imaged in the presence of 0 or 3 µM soluble tubulin. Shown are average values for each GMPCPP seed (dots), their mean and SD (error bars); **P < 0.01; ns—nonsignificant with one-way ANOVA followed by Tukey’s multiple comparison test; and n is the number of GMPCPP seeds analyzed for each data set. Data distribution was assumed to be normal, but this was not formally tested.

CEP97^CP110 forms caps at the plus ends of GMPCPP-stabilized MTs. (A) Kymographs showing GMPCPP-stabilized seeds (magenta) in absence (top row) or presence (bottom row) of 3 µM soluble tubulin labelled with TMR-tubulin (gray). (B) Slices through denoised tomograms containing plus ends of MT seeds in the absence or presence of 80 nM CEP97^CP110-GFP in the absence of free tubulin. (C and D) Average PF lengths (C) and average curvatures (D) of PFs with nonzero length for GMPCPP seeds imaged in the presence of 0 or 3 µM soluble tubulin. Shown are average values for each GMPCPP seed (dots), their mean and SD (error bars); **P < 0.01; ns—nonsignificant with one-way ANOVA followed by Tukey’s multiple comparison test; and n is the number of GMPCPP seeds analyzed for each data set. Data distribution was assumed to be normal, but this was not formally tested.

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