Figure S3.
Characterization of MT ends by cryo-ET. (A) Determination of MT polarity. For each MT: sum of slices containing the MT (top) and the same image Fourier filtered at origin (bottom). (B) Gallery of MT ends—plus and minus, capped by CEP97^CP110-GFP and uncapped, in the presence of 15 µM tubulin. Scale bar: 50 nm. (C) Sum of slices obtained from the tomograms rotated 90° to illustrate the end-on view of PF flares. Plus ends typically show clockwise twist pattern, while minus ends typically show counterclockwise pattern. The twist pattern is also observed for 13-PF MT ends.

Characterization of MT ends by cryo-ET. (A) Determination of MT polarity. For each MT: sum of slices containing the MT (top) and the same image Fourier filtered at origin (bottom). (B) Gallery of MT ends—plus and minus, capped by CEP97^CP110-GFP and uncapped, in the presence of 15 µM tubulin. Scale bar: 50 nm. (C) Sum of slices obtained from the tomograms rotated 90° to illustrate the end-on view of PF flares. Plus ends typically show clockwise twist pattern, while minus ends typically show counterclockwise pattern. The twist pattern is also observed for 13-PF MT ends.

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