Subcellular localization of GFP-tagged CP110 and its fragments, CEP97, and CEP97^CP110 chimera. (A) U2OS transiently transfected with the indicated GFP-tagged constructs were fixed and stained with antibodies against CEP192 (magenta), GFP (green), and tyrosinated tubulin (gray). White box highlights region with centrioles, which are enlarged in zoom. (B) U-ExM images of centrioles from U2OS cells overexpressing the indicated constructs and stained for acetylated tubulin (blue), CP110 (magenta), and GFP (green). CP110 full-length and CEP97^CP110 both localize to the distal cap of the mother centriole (white arrowhead) and distal cap of the daughter centriole.