Structural analysis of TPX2 proteins. (A) Sequence alignment of TPX2 in five vertebrate species and secondary structure analysis of human TPX2 using Jalview. The α-helix is marked with a red bar under each repeat. Critical MT-binding residues within human TPX2 repeats identified in this study are marked with red arrowheads. (B) Sequence alignment of 10 tandem repeats in human TPX2. The red bar below indicates a summary of predictions for the α-helix.