Figure S4.
Yeast GFP-Mdm33 concentrates at focal structures at steady state. (A) A maximum intensity projection of a deconvolved fluorescence microscopy image is shown of wild-type yeast cells expressing GFP-Mdm33 from the endogenous chromosomal locus and co-expressing mito-dsRed. Dashed lines indicate cell outlines. Yellow arrows mark sites of GFP-Mdm33 focal accumulation. (B) A quantification of the indicated number of GFP-Mdm33 focal enrichments detected per cell as in A. Data shown represent 75 cells per condition in each of three independent experiments, and bars indicate SEM. Scale bars: 3 µm.

Yeast GFP-Mdm33 concentrates at focal structures at steady state. (A) A maximum intensity projection of a deconvolved fluorescence microscopy image is shown of wild-type yeast cells expressing GFP-Mdm33 from the endogenous chromosomal locus and co-expressing mito-dsRed. Dashed lines indicate cell outlines. Yellow arrows mark sites of GFP-Mdm33 focal accumulation. (B) A quantification of the indicated number of GFP-Mdm33 focal enrichments detected per cell as in A. Data shown represent 75 cells per condition in each of three independent experiments, and bars indicate SEM. Scale bars: 3 µm.

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