Persistent recruitment of vinculin to apical junctions via α-cat ΔM2-3 mutant impacts early mitotic rounding but not cytokinesis. (A) Confocal, maximum z-projection images (inverted grayscale) and x-z slices showing that α-cat-ΔM2,3 constitutively and symmetrically recruits Vinculin to cell-cell junctions (red arrowheads), GFP-α-cat (green), LZTS2 (magenta). Scale bar, 5 μm. (B) Confocal, maximum z-projection images (inverted grayscale) show that while α-cat-ΔM2,3 limits mitotic rounding relative to α-cat-WT-expressing cells (yellow arrows; mitotic cell shape trace), binucleation rates are similar (see Fig. 10).