Figure 8. LZTS2 recruitment by α-cat ΔM1... | Rockefeller University Press

Figure 8.

$LZTS2 recruitment by α-cat ΔM1 is more uniform and refractory to myosin-inhibition compared with WT α-cat. (A and C) Coverslip grown MDCK cells treated with blebbistatin (100 μM, 3-h) reduced LZTS2 (magenta) localization to bicellular contacts in GFP-α-cat-WT monolayer (A), but not GFP-α-cat ΔM1 monolayer (C). Confocal en face views (maximum z-projections) are shown. Scale bar, 10 μm. Note LZTS2 becomes more evenly distributed across bi- and tri-cellular junctions by the α-cat ΔM1 mutant WT α-cat. (B and D) Normalized intensity profiles of GFP-α-cat (green) and LZTS2 (magenta) based on line scans (width: three pixels) performed across cytosol to bicellular junctions (line scan average of ∼10 junctions/construct and condition (individual dots shown). Refer to the image caption for details.$

LZTS2 recruitment by α-cat ΔM1 is more uniform and refractory to myosin-inhibition compared with WT α-cat. (A and C) Coverslip grown MDCK cells treated with blebbistatin (100 μM, 3-h) reduced LZTS2 (magenta) localization to bicellular contacts in GFP-α-cat-WT monolayer (A), but not GFP-α-cat ΔM1 monolayer (C). Confocal en face views (maximum z-projections) are shown. Scale bar, 10 μm. Note LZTS2 becomes more evenly distributed across bi- and tri-cellular junctions by the α-cat ΔM1 mutant WT α-cat. (B and D) Normalized intensity profiles of GFP-α-cat (green) and LZTS2 (magenta) based on line scans (width: three pixels) performed across cytosol to bicellular junctions (line scan average of ∼10 junctions/construct and condition (individual dots shown).