α-cat-ΔM1 preferentially recruits LZTS2 to cell contacts over wild-type and other α-cat mutants. (A) Confocal en face views (maximum z-projection; inverted grayscale) and x-z stacks showing α-cat-ΔM1 enriches LZTS2 at cellular junctions, α-cat (green), LZTS2 (magenta), Scale bar, x-y view; 10 μm, x-z view, 5 μm. Yellow arrows indicate plane of x-z section. Representative images from four independent experiments are shown. (B) Normalized intensity profiles of GFP-α-cat (green) and LZTS2 (magenta) based on line scans (width: three pixels) performed across cytosol to bicellular junctions. Line scan average of five junctions/construct. (C) Automated quantification of LZTS2 junction/cytosol ratio (upper graph), one way ANOVA, *P < 0.05, paired with automated quantification of GFP-α-cat junction intensity (lower graph) as in Materials and methods. Automated quantification based on five randomly selected 90 × 90 μm FOVs. Error bars reflect ± SD. (D) Immunoblots of total cell extracts showing the expression levels of different GFP-α-cat constructs (red bands) with tubulin loading control (green). (E) Schematics of GFP-α-cat constructs. Source data are available for this figure: SourceData F7.