Figure 4.
α-cat M-domain missense mutants that cause eye dystrophy promote wound-proximal binucleation. (A) Schematic of α-cat missense mutants used for analysis. (B) Immunoblots of total cell extracts showing similar expression levels of α-cat missense mutants. (C) Graph quantification of wound proximal binucleation rate (representative images in D). Mean ± SD, two-tailed unpaired t test, ***P < 0.001, ****P < 0.0001. Data from three independent bio-replicates, indicated by blue, gray and black symbol colors. (D) Inverted-grayscale images showing MDCK α-cat KO cells expressing GFP-α-cat-E307K, GFP-αCat-L436P, GFP-αCat-R551A are prone to binucleation post-scratch wounding. GFP- α-cat (green), F-actin (Phalloidin; cyan), nuclei (magenta), binucleated cells (outlined, yellow dashed line). Red arrows denote touching nuclei typical to polyploid cells. Scale bar, 50 μm. Source data are available for this figure: SourceData F4. Refer to the image caption for details.

α-cat M-domain missense mutants that cause eye dystrophy promote wound-proximal binucleation. (A) Schematic of α-cat missense mutants used for analysis. (B) Immunoblots of total cell extracts showing similar expression levels of α-cat missense mutants. (C) Graph quantification of wound proximal binucleation rate (representative images in D). Mean ± SD, two-tailed unpaired t test, ***P < 0.001, ****P < 0.0001. Data from three independent bio-replicates, indicated by blue, gray and black symbol colors. (D) Inverted-grayscale images showing MDCK α-cat KO cells expressing GFP-α-cat-E307K, GFP-αCat-L436P, GFP-αCat-R551A are prone to binucleation post-scratch wounding. GFP- α-cat (green), F-actin (Phalloidin; cyan), nuclei (magenta), binucleated cells (outlined, yellow dashed line). Red arrows denote touching nuclei typical to polyploid cells. Scale bar, 50 μm. Source data are available for this figure: SourceData F4.

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