Figure 2.
Persistent coupling of α-cat to F-actin promotes binucleation via cytokinesis failure. (A) Live imaging analysis of MDCK α-cat KO cells restored with α-cat-WT(Top) or α-cat-H0-ABD+(Bottom) during mitosis phases (metaphase, anaphase, telophase and cytokinesis). Time stamp in minutes (‘) shown in white; GFP-α-cat (green), nuclei (magenta), white arrowheads indicate dividing cells. A binucleated cell after cytokinesis failure is shown (inset with yellow arrowheads shows two nuclei in one cell). See also Video 1. Scale bar, 30 μm. (B) Quantification of α-cat-WT or αCat-H0-ABD+ cells (N = 30); *P < 0.05 by unpaired t test. (C) Graph shows percentage of cell divisions and cytokinesis failures over five fields of view; *P < 0.05, unpaired t test. Graph indicates mean ± SD. Refer to the image caption for details.

Persistent coupling of α-cat to F-actin promotes binucleation via cytokinesis failure. (A) Live imaging analysis of MDCK α-cat KO cells restored with α-cat-WT(Top) or α-cat-H0-ABD+(Bottom) during mitosis phases (metaphase, anaphase, telophase and cytokinesis). Time stamp in minutes (‘) shown in white; GFP-α-cat (green), nuclei (magenta), white arrowheads indicate dividing cells. A binucleated cell after cytokinesis failure is shown (inset with yellow arrowheads shows two nuclei in one cell). See also Video 1. Scale bar, 30 μm. (B) Quantification of α-cat-WT or αCat-H0-ABD+ cells (N = 30); *P < 0.05 by unpaired t test. (C) Graph shows percentage of cell divisions and cytokinesis failures over five fields of view; *P < 0.05, unpaired t test. Graph indicates mean ± SD.

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