TEM4 depletion slows microtubule regrowth and results in the spindle. (A) HeLa-T-REx control or TEM4-depleted cells were treated for 3 h with 3 μM nocodazole. Nocodazole was washed out and cells were incubated with fresh media for the indicated time points. Cells were fixed with PTEMF buffer and stained with CREST serum (blue), anti-α-tubulin (green), and anti-γ-tubulin (red). Scale bar = 5 μm. (B) Cells treated as in A. Representative images of mitotic spindle phenotypes observed after spindle reformation. Scale bar = 5 μm.