Delayed mitotic entry and in the absence of TEM4. (A) Representative stills of time-lapse movies of control, TEM4-21, and TEM4-22 cells 72 h after induction. For imaging, cells were synchronized in mitosis after release from the double thymidine block and incubated with SiR-DNA and SPY555-tubulin to visualize the chromatin and the mitotic spindle. Scale bar = 5 μm. (B) The cumulative mitotic index was measured from time-lapse movies of HeLa-T-REx cells depleted of TEM4 shown in A. Data shown is mean ± SD (from ∼300 cells in total, n = 3 independent experiments).