The NTD of NSP3 directly interacts with NSP12. (A) Schematic representation of the structures of the NSP3 truncations. (B) Confocal microscopy analysis showing the colocalization of NSP12-mCherry with truncated Flag-NSP3/NSP4-tagBFP–induced punctate structures in HeLa cells. Scale bar, 10 μm. (C) In vitro pull-down analysis of the interaction between NTD of NSP3 and NSP12. Purified NTD of NSP3 and NSP12 proteins were subjected to pull-down assay and pull-down samples by Flag antibody were analyzed by IB with the indicated antibodies. Flag-GST protein was used as a negative control. Input represents 5% of the total proteins used for pull-down. Molecular weights are in kDa. n = 3 independent experiments. (D) Schematic representation of the structures of the NSP12 truncations. (E) Confocal microscopy analysis showing the colocalization of truncated NSP12-mCherry with GFP-NSP3/NSP4-tagBFP–induced punctate structures in HeLa cells. Scale bar, 10 μm. Source data are available for this figure: SourceData F3.