Other NSPs are not recruited to the NSP3/4-induced DMVs, related to Fig. 2 . (A) Confocal microscopy analysis of the localization of NSP1-mCherry, NSP2-mCherry, GFP-NSP3, NSP4-GFP, NSP5-mCherry, mCherry-NSP6, NSP7-mCherry, NSP8-mCherry, NSP9-mCherry, NSP10-mCherry, NSP11-mCherry, NSP12-mCherry, NSP13-mCherry, NSP14-mCherry, NSP15-mCherry, and NSP16-mCherry in HeLa cells. Scale bar, 10 μm. (B) Confocal microscopy analysis of the co-localization of Flag-NSP3/NSP4-GFP puncta with NSP1-mCherry, NSP2-mCherry, NSP5-mCherry, NSP7-mCherry, NSP8-mCherry, NSP9-mCherry, NSP10-mCherry, NSP11-mCherry, NSP13-mCherry, NSP14-mCherry, NSP15-mCherry, or NSP16-mCherry in HeLa cells. Scale bar, 10 μm.