Screening procedure of the parameter sets and analyses of the simulation results. (A) Flowchart for screening oscillation features in the simulation results. There are four filters in this flowchart. Corresponding figures to illustrate or demonstrate the results are also labeled. a In practice, at least one length with a period greater than zero. b The set with only one length with a period greater than zero was removed. (B) Demonstration of the concentration profile of membrane-bound MinD (Cd + Cde) and identification of IRatio. The left intensity profile (red-hued hollow markers) was collected at 48.88 s when the maximal intensity occurred at the left pole. Each data point is normalized against the maximum intensity on this profile. Similarly, the right profile (blue-hued hollow markers) was collected at 73.9 s and processed. The resulting left and right profiles are symmetrical, with less than a 0.02% difference between them. The Imax and Imin values were identified from the fitted curve for calculation of the theoretical IRatio. Here, the profile is fitted from the maximum to the minimum intensity using an exponential equation y = 0.96exp(−2.89x)+0.17, with λN = 2.89. (C) Demonstration of the length-dependent changes in λN and the biphasic mode. The incremental λN values are fitted with a linear function λN = aL + b or λN = c, where L is the length. (D) Kymographs of oscillating membrane-bound MinD (Cd + Cde) between 40 and 140 s. (a) Typical pole-to-pole oscillation. (b) Oscillation starts late during simulation. (c and d) Irregular oscillation.
