Characteristics of the MinD concentration gradient. (A–F) The cells were grown in 0.4% (A–C) or 0% glucose (D–F). (A and D) Kymographs of the fitted intensity profiles. The red line in each graph indicates the median cell length in the population. (B and E) The correlation plots of the wave slope (λ_N) against cell length. (C and F) The correlation plots of the wave slope (I_Ratio) against the cell length. In B, C, E, and F, the blue dashed line indicates the λ_N or I_Ratiovalue at the median cell length. The red dashed lines are obtained by simple linear regression. Correlations were calculated using the nonparametric Spearman correlation method with 95% confidence intervals. R², goodness of fit; r, Spearman coefficient; P: two-tailed probability; n, population size. This analysis was performed using the same image datasets as in Fig. 1, D–G, with an additional intensity threshold applied to filter the datasets for the estimation of λ_N.