Number of MinD molecules throughout the cell cycle. (A) The bulk fluorescence intensity of sfGFP-MinD (diamond, red-hued lines) increased over time, while the intensity per unit area (circle, blue-hued lines) exhibited only moderate fluctuations. Data from paired daughter cells are distinguished by different colors. This plot includes partial data of a dividing cell from time-lapse image sequences acquired over a 5-h period at 15-min intervals. (B) The number of molecules in a single cell (red-hued line) and per unit area (µm2; blue-hued line) were plotted against time and fitted by simple linear regression (n = 100). Pooled data from several image fields were used for the analysis. The solid line represents the best-fit regression line, while the dashed lines represent the errors. Fast-growing cells that divided within five frames were excluded. (C) The number of molecules (red-hued line) and the number per unit area (µm2; blue-hued line) within 200 nm of the cell center were estimated based on the fraction from the MinD gradient profile (inset) before being plotted against time and fitted using simple linear regression (n = 100). The solid line represents the best-fit regression line, while the dashed lines represent the errors.
