Examination of FtsA localization, which marks the division site, in relation to cell length and MinD oscillation period in E. coli strain FW1541. (A) Representative images demonstrating simultaneous observation of FtsA-mScarlet-I, sfGFP-MinD, and cell morphology. (B and C) The ring-like structure of FtsA-mScarlet-I had a relative position of 47.5 ± 2.1% from either pole (B), regardless of the cell length and the period of the MinD oscillations (C). The cell center is set at position 0.5 for normalization of the FtsA position. Pooled data from several image fields were used for the analysis. The Spearman nonparametric correlation method was used to calculate the correlation with 95% confidence intervals. The red dashed line represents the best-fit linear regression line. n, population size; R², goodness of fit; r, Spearman coefficient; P: two-tailed probability.