The oscillation period and velocity before and after division demonstrated that the oscillation velocity decreased in newborn cells. In some cases, the fluorescence in one of the two newborn cells was too weak to be studied. The numbers on the x-axis indicate the cell lineage. To image MinD oscillations before and after cell division, cells mounted on an agarose pad were initially imaged for 10 min, then incubated on the heating stage of the microscope at 30°C for 2 h before being imaged again for 10 min. Divided cells were selected for analysis.