Figure S4.
The effects of THZ1 and BafA1 on cilia base TTLL5/6-YFP and the effects of cilia-trapped YFP, CDK6, or CyclinD3-CDK6 on total FIP5 protein abundance. (A) Autophagy inhibitor bafilomycin-A1 (BafA1) prevents the loss of TTLL5/6-YFP at the cilia base in THZ1-treated cells. Scale bar: 2 μm. TTLL5/6-YFP expressing RPE1 cells were pretreated with or without BafA1 (10 nM) for 1 h followed by 2 h THZ1 treatment. (B) Ciliary CDK6 does not affect the protein level of FIP5. YFP control, CDK6 or CyclinD3-CDK6 were overexpressed at cilia base. The protein level of FIP5 was accessed by western blotting. The molecular weigth standards (kD) are labeled on the right. Source data are available for this figure: SourceData FS4.

The effects of THZ1 and BafA1 on cilia base TTLL5/6-YFP and the effects of cilia-trapped YFP, CDK6, or CyclinD3-CDK6 on total FIP5 protein abundance. (A) Autophagy inhibitor bafilomycin-A1 (BafA1) prevents the loss of TTLL5/6-YFP at the cilia base in THZ1-treated cells. Scale bar: 2 μm. TTLL5/6-YFP expressing RPE1 cells were pretreated with or without BafA1 (10 nM) for 1 h followed by 2 h THZ1 treatment. (B) Ciliary CDK6 does not affect the protein level of FIP5. YFP control, CDK6 or CyclinD3-CDK6 were overexpressed at cilia base. The protein level of FIP5 was accessed by western blotting. The molecular weigth standards (kD) are labeled on the right. Source data are available for this figure: SourceData FS4.

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