Inhibition of CDK6 abolishes cilia disassembly. (A and B) hTERT-RPE-1 cells were transfected with siRNA for 48 h, followed by serum starvation and Abemaciclib (500 nM) treatment for 24 h. After that, cells were cultured in the medium containing 20% FBS for the indicated times. The cilia length was measured using ARL13B as a marker at indicated times. Quantified data are presented as mean ± SEM. Statistical analyses were performed by one-way ANOVA analyses with Tukey’s post-hoc test for multiple comparisons. N ≥ 50 cilia. **P < 0.01; ***P < 0.001. n.s: not significant.