Inhibition of CDK7 or CDK6 does not affect tubulin glutamylation and acetylation in cytoplasm. (A and B) THZ1 or Abemaciclib does not affect cytoplasmic tubulin glutamylation. (C and D) Knockdown of CDK7 or CDK6 does not affect cytoplasmic tubulin glutamylation. (E) The total levels of glutamylated tubulin and acetylated tubulin in control and CDK6^−/− RCTE cells were assessed by western bolting. The molecular weigth standards (kD) are labeled on the right. (F) Knockdown of CDK7 or CDK6 does not affect the axonemal acetylation-to-cilium length ratio in RPE1 cells. Cilia length was measured using ARL13B as a cilia marker. Quantified data are presented as mean ± SEM. N ≥ 30 Cilia. Statistical analyses were performed by one-way ANOVA analyses with Tukey’s post-hoc test for multiple comparisons. n.s: not significant. Scale bars: 5 μm. GT335 antibody labels all forms of glutamylated tubulin. PolyE antibody only labels polyglutamylated tubulin (≥3E). Source data are available for this figure: SourceData FS2.