Kinase inhibitors screen to identify regulators of axoneme polyglutamylation. RPE-1 cells were treated with each kinase inhibitor for 24 h (1 μM) in serum-free medium, and the length of glutamylated axoneme was measured. The positive hits that affect axoneme polyglutamylation are presented. Quantified data are presented as mean ± SEM. N ≥ 30 Cilia.