Related to Fig. 7 . Localization of MYR::KNL-1 and expression of MYR::KNL-1 variants in the PVD. (A) Schematic with arrowheads highlighting the actin clusters (left above) and image of MYR::KNL-1::TagBFP induced actin clusters (left below). Insets on the right show images of Lifeact::mKate2 (magenta), the plasma membrane labeled with mNeonGreen(mNG)::PH (green) and MYR::KNL-1::TagBFP (blue) in three different actin clusters. Scale bar, 5 µm. (B) Quantification of KNL-1::TagBFP fluorescence intensity in the cell body normalized to maximum and minimum intensities in indicated conditions. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Kruskal–Wallis test followed by Dunn’s test.