Figure 5.
The PVD actin cytoskeleton is affected by KNL-1 degradation. (A) Images showing actin distribution (gray at the top, magenta at the bottom) in the PVD cell body and dendrites (green). Actin is visualized using Lifeact::mKate2, and the plasma membrane is labeled with mNeonGreen(mNG)::PH. Scale bar, 10 µm. (B) Images highlighting actin distribution in the cell body (CB) and proximal anterior primary dendrite in control and KNL-1 DEG animals. Scale bar, 5 µm. (C) Box and whisker plot showing quantification of the mean Lifeact::mKate2 fluorescence intensity along a three-pixel wide line along the cell body (CB) periphery. n represents the number of animals. Whiskers represent minimum and maximum while the bar represents the first, second, and third quartiles. P values from Mann–Whitney test. (D) Box and whisker plot showing quantification of maximum Lifeact::mKate2 fluorescence intensity inside the cell body. n represents the number of animals. Whiskers represent minimum and maximum whilst bar represents first, second, and third quartiles. P values from unpaired t test. (E) Box and whisker plot showing quantification of mean Lifeact::mKate2 fluorescence intensity of a cross section of the 1° dendrite 10 µm anterior to the cell body along a three-pixel-wide line. n represents the number of animals. Whiskers represent minimum and maximum whilst bar represents first, second, and third quartiles. P values from Mann–Whitney test. (F) Images of the anterior primary dendrite labeled with mNG::PH in control and KNL-1 DEG, in late L3 larval stage animals. Orange arrowheads indicate filopodial like membrane protrusions. Scale bar, 5 µm. (G) Quantification of the number of membrane protrusions appearing from the anterior primary dendrite over a 3-h time interval. n represents the number of animals. Error bars represent mean ± 95% CI. P values from unpaired t test.

The PVD actin cytoskeleton is affected by KNL-1 degradation. (A) Images showing actin distribution (gray at the top, magenta at the bottom) in the PVD cell body and dendrites (green). Actin is visualized using Lifeact::mKate2, and the plasma membrane is labeled with mNeonGreen(mNG)::PH. Scale bar, 10 µm. (B) Images highlighting actin distribution in the cell body (CB) and proximal anterior primary dendrite in control and KNL-1 DEG animals. Scale bar, 5 µm. (C) Box and whisker plot showing quantification of the mean Lifeact::mKate2 fluorescence intensity along a three-pixel wide line along the cell body (CB) periphery. n represents the number of animals. Whiskers represent minimum and maximum while the bar represents the first, second, and third quartiles. P values from Mann–Whitney test. (D) Box and whisker plot showing quantification of maximum Lifeact::mKate2 fluorescence intensity inside the cell body. n represents the number of animals. Whiskers represent minimum and maximum whilst bar represents first, second, and third quartiles. P values from unpaired t test. (E) Box and whisker plot showing quantification of mean Lifeact::mKate2 fluorescence intensity of a cross section of the 1° dendrite 10 µm anterior to the cell body along a three-pixel-wide line. n represents the number of animals. Whiskers represent minimum and maximum whilst bar represents first, second, and third quartiles. P values from Mann–Whitney test. (F) Images of the anterior primary dendrite labeled with mNG::PH in control and KNL-1 DEG, in late L3 larval stage animals. Orange arrowheads indicate filopodial like membrane protrusions. Scale bar, 5 µm. (G) Quantification of the number of membrane protrusions appearing from the anterior primary dendrite over a 3-h time interval. n represents the number of animals. Error bars represent mean ± 95% CI. P values from unpaired t test.

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