Figure S4.
Related toFig. 5. Analysis of membrane and F-actin dynamics in control and KNL-1 degraded animals. (A) Images of the PVD cell body (CB) and proximal regions in control and KNL-1 DEG labelled using mScarlet-I(mSca-I)::PH membrane marker at the L4 stage. Scale bar, 5 µm. (B) Quantification of the area of the cell body in control and KNL-1 DEG animals. n represents number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (C) Snapshots of time-lapse imaging of 1° dendrite branch dynamics using mNeonGreen(mNG)::PH membrane marker in control and KNL-1 DEG animals at the late L3 stage. Yellow arrowheads indicate already formed protrusions. White asterisks indicate newly extending protrusions. Yellow asterisks indicate the misguided protrusions that run parallel to the 1° dendrite. Scale bar, 5 µm. (D) Images of the membrane of the primary dendrite and the microtubule cytoskeleton labeled using mSca-I::PH membrane marker and endogenous GFP::TBA-1, respectively, in control and KNL-1 DEG animals at the L3 stage. Yellow arrowheads highlight membrane protrusions. Scale bar, 5 µm. (E) Box and whisker plot showing quantification of the mean intensity of GFP::TBA-1 within the membrane protrusions along the primary dendrite 50 µm anterior to the cell body. n represents the number of animals. Whiskers represent minimum and maximum while bar represents first, second, and third quartiles. P values from Mann–Whitney test. (F) Schematic and images of the anterior dorsal menorah proximal to the cell body showing the actin distribution at the 4° dendrites in control and KNL-1 DEG animals. Actin and membrane are labeled using Lifeact::mKate2 and mNG::PH, respectively. Scale bar, 5 µm. (G) Box and whisker plot showing quantification of Lifeact::mKate2 fluorescence intensity along a two-pixel wide line along 4°dendrites of the first anterior menorah. n represents the number of animals. Whiskers represent minimum and maximum whilst bar represents first, second, and third quartiles. P values from unpaired t test.

Related to Fig. 5 . Analysis of membrane and F-actin dynamics in control and KNL-1 degraded animals. (A) Images of the PVD cell body (CB) and proximal regions in control and KNL-1 DEG labelled using mScarlet-I(mSca-I)::PH membrane marker at the L4 stage. Scale bar, 5 µm. (B) Quantification of the area of the cell body in control and KNL-1 DEG animals. n represents number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (C) Snapshots of time-lapse imaging of 1° dendrite branch dynamics using mNeonGreen(mNG)::PH membrane marker in control and KNL-1 DEG animals at the late L3 stage. Yellow arrowheads indicate already formed protrusions. White asterisks indicate newly extending protrusions. Yellow asterisks indicate the misguided protrusions that run parallel to the 1° dendrite. Scale bar, 5 µm. (D) Images of the membrane of the primary dendrite and the microtubule cytoskeleton labeled using mSca-I::PH membrane marker and endogenous GFP::TBA-1, respectively, in control and KNL-1 DEG animals at the L3 stage. Yellow arrowheads highlight membrane protrusions. Scale bar, 5 µm. (E) Box and whisker plot showing quantification of the mean intensity of GFP::TBA-1 within the membrane protrusions along the primary dendrite 50 µm anterior to the cell body. n represents the number of animals. Whiskers represent minimum and maximum while bar represents first, second, and third quartiles. P values from Mann–Whitney test. (F) Schematic and images of the anterior dorsal menorah proximal to the cell body showing the actin distribution at the 4° dendrites in control and KNL-1 DEG animals. Actin and membrane are labeled using Lifeact::mKate2 and mNG::PH, respectively. Scale bar, 5 µm. (G) Box and whisker plot showing quantification of Lifeact::mKate2 fluorescence intensity along a two-pixel wide line along 4°dendrites of the first anterior menorah. n represents the number of animals. Whiskers represent minimum and maximum whilst bar represents first, second, and third quartiles. P values from unpaired t test.

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