Related to Fig. 4 . DMA-1 distribution, microtubule organization, and stability after degradation of KNL-1 in the PVD. (A) Localization of the synaptic marker RAB-3 in control and KNL-1 DEG. Orange arrowheads indicate example RAB-3 puncta. RAB-3 puncta are predominantly enriched in the axon in both conditions. Scale bar, 5 µm. CB, cell body. (B) Localization of the dendrite guidance receptor, DMA-1 in control and KNL-1 DEG at the L4 larval stage. (Left) Merged images of DMA-1::mKate2 with mNeonGreen(mNG)::PH membrane marker and (Right) DMA-1::mKate2 for the indicated conditions. DMA-1 is restricted to dendrite structures and no axonal signal for DMA-1 is visible. Purple arrowheads indicate example DMA-1 puncta. Scale bar, 5 µm. (C) Images display expression of GFP::TBA-1 driven by des-2 promoter in control and KNL-1 DEG animals. GFP::TBA-1 is predominantly localized to the 1° dendrite relative to the 2° and 3° dendrite branches. Scale bar, 10 µm. (D) Still images of the PVD anterior primary dendrite expressing endogenous GFP::TBA-1 in control and KNL-1 DEG animals (top). Scale bar, 5 µm. The dashed rectangles indicate regions that were photobleached in the FRAP experiments. Pre-bleach and post-bleach images of GFP::TBA-1 in control and KNL-1 DEG animals (bottom) Scale bar, 5 μm.