Figure 3.
KNL-1 is important for proprioceptive behavior and PVD neuron maintenance. (A) Cartoon representation of animal movement, where the arrow represents the sinusoidal track that the animal leaves behind, defined by the wavelength and amplitude (top). Images show the tracks of control, KNL-1 DEG and Δdma-1 animals. The control worms form sinusoidal tracks while the amplitude and wavelength of these tracks were diminished in KNL-1 DEG and Δdma-1 animals. Scale bar, 20 µm. (B and C) Quantification of the wavelength and amplitude of the tracks by animals in indicated conditions. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Kruskal–Wallis test followed by Dunn’s test (B) or one-way ANOVA test followed by Tukey’s test (C). (D) The plot represents the number of animals responding to harsh touch in the indicated conditions. The experiment was conducted in mec-4(e1611) mutant background, which lacks touch receptor neurons. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Kruskal–Wallis test followed by Dunn’s test. (E) Images of PVD dendrite organization at the L4 stage in control and KNL-1 DEG animals. Yellow arrowheads indicate the bead-like blebs in the plasma membrane of PVD neurons. Scale bar, 5 µm. (F) Quantification of the bead-like blebs in control and KNL-1 DEG animals. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (G) Images show PVD cell body and dendrites using mNeonGreen(mNG)::PH marker (green) and autophagosomes using mScarlet-I(mSca-I)::LGG-1 marker (magenta) in the indicated conditions. Yellow arrowheads indicate the appearance of LGG-1 puncta along the dendrites of KNL-1 DEG animals. Scale bar 10 µm. (H) A zoomed image of the PVD dendrite showing the plasma membrane in green and autophagosomes in magenta. Arrowhead highlights mSca-I::LGG-1 puncta (top). The plot below is the quantification of mSca-I::LGG-1 puncta within the dendrites in both control and KNL-1 DEG animals. n represents the number of animals. Error bars represent mean ± 95% CI. P values from unpaired t test. Scale bar 5 µm.

KNL-1 is important for proprioceptive behavior and PVD neuron maintenance. (A) Cartoon representation of animal movement, where the arrow represents the sinusoidal track that the animal leaves behind, defined by the wavelength and amplitude (top). Images show the tracks of control, KNL-1 DEG and Δdma-1 animals. The control worms form sinusoidal tracks while the amplitude and wavelength of these tracks were diminished in KNL-1 DEG and Δdma-1 animals. Scale bar, 20 µm. (B and C) Quantification of the wavelength and amplitude of the tracks by animals in indicated conditions. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Kruskal–Wallis test followed by Dunn’s test (B) or one-way ANOVA test followed by Tukey’s test (C). (D) The plot represents the number of animals responding to harsh touch in the indicated conditions. The experiment was conducted in mec-4(e1611) mutant background, which lacks touch receptor neurons. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Kruskal–Wallis test followed by Dunn’s test. (E) Images of PVD dendrite organization at the L4 stage in control and KNL-1 DEG animals. Yellow arrowheads indicate the bead-like blebs in the plasma membrane of PVD neurons. Scale bar, 5 µm. (F) Quantification of the bead-like blebs in control and KNL-1 DEG animals. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (G) Images show PVD cell body and dendrites using mNeonGreen(mNG)::PH marker (green) and autophagosomes using mScarlet-I(mSca-I)::LGG-1 marker (magenta) in the indicated conditions. Yellow arrowheads indicate the appearance of LGG-1 puncta along the dendrites of KNL-1 DEG animals. Scale bar 10 µm. (H) A zoomed image of the PVD dendrite showing the plasma membrane in green and autophagosomes in magenta. Arrowhead highlights mSca-I::LGG-1 puncta (top). The plot below is the quantification of mSca-I::LGG-1 puncta within the dendrites in both control and KNL-1 DEG animals. n represents the number of animals. Error bars represent mean ± 95% CI. P values from unpaired t test. Scale bar 5 µm.

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