Related to Fig. 2 . Effects of degradation of KMN network on PVD morphology. (A) Schematic indicating the experimental design for degrading AID::KNL-1. To obtain synchronized L1 animals, embryos from control or AID::KNL-1 adult worms expressing PVD-specific TIR1 transgene were isolated by treating the worms with bleach and hatched for 24 h on plates without food. The arrested L1 larvae were placed on a plate with food until they reached the L2 stage (20 h). The late L2 stage animals were transferred to plates with food and auxin and grown until they reached L3 (16 h) or L4 stage (28 h). The late L4 stage animals, which have fully developed menorahs, were imaged to analyze the PVD morphology. (B and C) Quantification of the number of 2° branches and the number of protrusions for the indicated conditions. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Kruskal–Wallis test followed by Dunn’s test. (D and E) Quantification of PVD dendrite architecture including number of 4° branches and overlaps between menorahs in control and KNL-1 DEG in anterior and posterior dendrites. n represents number of animals. Error bars represent mean ± 95% CI. P values from one-way ANOVA test followed by Tukey’s test (D) or Kruskal–Wallis test followed by Dunn’s test (E). (F) Quantification of the number of 2° branches in the PVD in GFP and KNL-1::GFP overexpressing animals. n represents the number animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (G) Schematic of the KMN network complexes (KNL1/Mis12/Ndc80) and their components (top). Images of PVD dendrite organization in control, KNL-1 DEG, NDC-80 DEG & KNL-3 DEG animals with mScarlet-I(mSca-I)::PH membrane marker (bottom). Scale bar, 10 µm. (H and I) Quantification of the number of 4° branches and overlaps between menorahs for the indicated conditions. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Kruskal–Wallis test followed by Dunn’s test.