Figure 2.
KNL-1 is required for proper dendrite branching of the PVD neuron. (A) Approach employed for the degradation of endogenously tagged AID::KNL-1 in the PVD neuron (top). Images below show GFP::KNL-1 signal (gray) within the PVD cell body (magenta) at the late L2 larval stage, in the absence (Control DEG) and presence of auxin (KNL-1 DEG). The graph on the right shows the mean fluorescence intensity of the GFP signal in the cell body for control and KNL-1 DEG conditions. n represents the number of animals. Error bars indicate mean ± 95% confidence interval (CI). P values from unpaired t test. Scale bar, 1 µm. (B) Images of PVD dendrite organization at the L4 larval stage in the control and KNL-1 DEG animals. These images show both the anterior and posterior dendrites extending from the cell body within a 100 µm region. Scale bar, 10 µm. (C and D) Quantification of PVD dendrite architecture determining the number of 4° branches and overlaps between menorahs in control and KNL-1 DEG. n represents the number of animals. Error bars represent mean ± 95% CI. P values from unpaired t test (C) or Mann–Whitney test (D). (E) Images of PVD dendrites in GFP (control) and KNL-1::GFP overexpressing animals. In both conditions the transgenes were overexpressed as an extrachromosomal array under a PVD specific promoter. n represents the number of animals. Scale bars, 10 µm. (F) Quantification of the number of 4° branches in the PVD in GFP and KNL-1::GFP overexpressing animals. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (G) Time-lapse imaging of 3° dendrite branch dynamics in control and KNL-1 DEG animals. Yellow arrowheads denote the contact sites between 3° dendrites of two adjacent menorahs. Scale bar, 5 µm. (H) Quantification of number of contacts made by tertiary dendrites of the PVD neuron over a 3-h time interval in the indicated conditions. n represents number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (I) Quantification of number of unresolved contacts (UCs) made by tertiary dendrites of the PVD neuron over a 3-h time interval in the indicated conditions. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (J) Quantification of time spent in contact by 3° dendrites of two adjacent menorahs. n represents the number of contacts. Error bars represent mean ± 95% CI. P values from Mann–Whitney test.

KNL-1 is required for proper dendrite branching of the PVD neuron. (A) Approach employed for the degradation of endogenously tagged AID::KNL-1 in the PVD neuron (top). Images below show GFP::KNL-1 signal (gray) within the PVD cell body (magenta) at the late L2 larval stage, in the absence (Control DEG) and presence of auxin (KNL-1 DEG). The graph on the right shows the mean fluorescence intensity of the GFP signal in the cell body for control and KNL-1 DEG conditions. n represents the number of animals. Error bars indicate mean ± 95% confidence interval (CI). P values from unpaired t test. Scale bar, 1 µm. (B) Images of PVD dendrite organization at the L4 larval stage in the control and KNL-1 DEG animals. These images show both the anterior and posterior dendrites extending from the cell body within a 100 µm region. Scale bar, 10 µm. (C and D) Quantification of PVD dendrite architecture determining the number of 4° branches and overlaps between menorahs in control and KNL-1 DEG. n represents the number of animals. Error bars represent mean ± 95% CI. P values from unpaired t test (C) or Mann–Whitney test (D). (E) Images of PVD dendrites in GFP (control) and KNL-1::GFP overexpressing animals. In both conditions the transgenes were overexpressed as an extrachromosomal array under a PVD specific promoter. n represents the number of animals. Scale bars, 10 µm. (F) Quantification of the number of 4° branches in the PVD in GFP and KNL-1::GFP overexpressing animals. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (G) Time-lapse imaging of 3° dendrite branch dynamics in control and KNL-1 DEG animals. Yellow arrowheads denote the contact sites between 3° dendrites of two adjacent menorahs. Scale bar, 5 µm. (H) Quantification of number of contacts made by tertiary dendrites of the PVD neuron over a 3-h time interval in the indicated conditions. n represents number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (I) Quantification of number of unresolved contacts (UCs) made by tertiary dendrites of the PVD neuron over a 3-h time interval in the indicated conditions. n represents the number of animals. Error bars represent mean ± 95% CI. P values from Mann–Whitney test. (J) Quantification of time spent in contact by 3° dendrites of two adjacent menorahs. n represents the number of contacts. Error bars represent mean ± 95% CI. P values from Mann–Whitney test.

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