Figure S1.
Related toFig. 1. PVD morphogenesis and KNL-1 localization. (A) Images show GFP1-10β control across anterior dendrite at the L3 stage. White arrow highlights 1° dendrite, which shows no detectable signal for GFP β1-10 in the absence of the complementing β11 strand. Pink circle highlights an autofluorescence granule. The images are maximum intensity projections of four z-planes spaced 0.5 µm apart. Scale bar, 5 µm. (B) Images (top) and linescan of the fluorescence intensity (bottom) showing GFP::KNL-1 and mScarlet-I (mSca-I) cytoplasmic probe distribution along the primary dendrite 50 µm anterior to the cell body at the L3 stage. The images are maximum intensity projections of three z-planes spaced 0.5 µm apart. Scale bar, 10 µm. (C) Image showing GFP::KNL-1 puncta distribution along 1° dendrite at the L3 stage (right). Scale bar, 1 µm. Graph showing linescan of the fluorescence intensity of GFP::KNL-1 (left). (D) Image shows the distribution of GFP::KNL-1 along the 3° dendrite at the early L4 stage. Scale bar, 2 µm. (E) Left: Schematic (top) and images (bottom) shows the localization of GFP::KNL-1 in the first menorah anterior to the cell body. Right: Insets of GFP::KNL-1 puncta (green) and mSca-I (magenta) at the 2° and 3° branch points. The images are maximum intensity projections of two z-planes spaced 0.5 µm apart. Scale bar, 1 µm. Only a subset of the branch point contains GFP::KNL-1 signal. (F) Quantification of GFP::KNL-1 and the mSca-I signal at the intersections of 2° and 3° in 41 animals including the ones without GFP::KNL-1. Dotted lines and shaded region represent mean ± SEM. Branch points show an increased intensity of both the cytoplasmic mSca-I and GFP::KNL-1 signal. (G) Quantification of the distribution of GFP::KNL-1 puncta at the branch points between 2° and 3° compared with the GFP β1-10, which was used as a negative control. GFP::KNL-1 positive branch points were analyzed only within the menorahs 50 µm anterior to the cell body. n represents the number of animals. Error bars indicate mean ± SD. (H) Left: Schematic (top) and images (bottom) show the localization of GFP::KNL-1 in the first menorah anterior to the cell body. Right: Insets of GFP::KNL-1 puncta (green) and mSca-I (magenta) at the 1° and 2° branch point. The images are maximum intensity projections of two z-planes spaced 0.5 µm apart. Scale bar, 1 µm. Only a subset of the branch point contains GFP::KNL-1 signal. (I) Quantification of GFP::KNL-1 and the mSca-I signal at the intersections of 1° and 2° in 36 animals including the ones without GFP::KNL-1. Dotted lines and shaded region represent mean ± SEM. Branch points show an increased intensity of both the cytoplasmic mSca-I and GFP::KNL-1 signal. (J) Quantification of the distribution of GFP::KNL-1 puncta at the branch points between 1° and 2° compared to the GFP β1-10 which was used as a negative control. GFP::KNL-1 positive branch points were analyzed only within the menorahs 50 µm anterior to the cell body. n represents the number of animals. Error bars indicate mean ± SD.

Related to Fig. 1 . PVD morphogenesis and KNL-1 localization. (A) Images show GFP1-10β control across anterior dendrite at the L3 stage. White arrow highlights 1° dendrite, which shows no detectable signal for GFP β1-10 in the absence of the complementing β11 strand. Pink circle highlights an autofluorescence granule. The images are maximum intensity projections of four z-planes spaced 0.5 µm apart. Scale bar, 5 µm. (B) Images (top) and linescan of the fluorescence intensity (bottom) showing GFP::KNL-1 and mScarlet-I (mSca-I) cytoplasmic probe distribution along the primary dendrite 50 µm anterior to the cell body at the L3 stage. The images are maximum intensity projections of three z-planes spaced 0.5 µm apart. Scale bar, 10 µm. (C) Image showing GFP::KNL-1 puncta distribution along 1° dendrite at the L3 stage (right). Scale bar, 1 µm. Graph showing linescan of the fluorescence intensity of GFP::KNL-1 (left). (D) Image shows the distribution of GFP::KNL-1 along the 3° dendrite at the early L4 stage. Scale bar, 2 µm. (E) Left: Schematic (top) and images (bottom) shows the localization of GFP::KNL-1 in the first menorah anterior to the cell body. Right: Insets of GFP::KNL-1 puncta (green) and mSca-I (magenta) at the 2° and 3° branch points. The images are maximum intensity projections of two z-planes spaced 0.5 µm apart. Scale bar, 1 µm. Only a subset of the branch point contains GFP::KNL-1 signal. (F) Quantification of GFP::KNL-1 and the mSca-I signal at the intersections of 2° and 3° in 41 animals including the ones without GFP::KNL-1. Dotted lines and shaded region represent mean ± SEM. Branch points show an increased intensity of both the cytoplasmic mSca-I and GFP::KNL-1 signal. (G) Quantification of the distribution of GFP::KNL-1 puncta at the branch points between 2° and 3° compared with the GFP β1-10, which was used as a negative control. GFP::KNL-1 positive branch points were analyzed only within the menorahs 50 µm anterior to the cell body. n represents the number of animals. Error bars indicate mean ± SD. (H) Left: Schematic (top) and images (bottom) show the localization of GFP::KNL-1 in the first menorah anterior to the cell body. Right: Insets of GFP::KNL-1 puncta (green) and mSca-I (magenta) at the 1° and 2° branch point. The images are maximum intensity projections of two z-planes spaced 0.5 µm apart. Scale bar, 1 µm. Only a subset of the branch point contains GFP::KNL-1 signal. (I) Quantification of GFP::KNL-1 and the mSca-I signal at the intersections of 1° and 2° in 36 animals including the ones without GFP::KNL-1. Dotted lines and shaded region represent mean ± SEM. Branch points show an increased intensity of both the cytoplasmic mSca-I and GFP::KNL-1 signal. (J) Quantification of the distribution of GFP::KNL-1 puncta at the branch points between 1° and 2° compared to the GFP β1-10 which was used as a negative control. GFP::KNL-1 positive branch points were analyzed only within the menorahs 50 µm anterior to the cell body. n represents the number of animals. Error bars indicate mean ± SD.

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