Figure 7.
Short-term and chronic plasticity of the AIS in AcD neurons. (A) Representative images of the AIS in nonAcD neurons upon induction of short-term (top row) and chronic (bottom row) plasticity. Neurons were treated with KCl for depolarization, NaCl as control and TTX for silencing of activity. Red arrowheads in upper row indicate the start and end of AIS. Red dashed line in bottom row indicates the start of the axon. Scale bar is 10 µm. (B) Measurement of AIS length and AIS distance in nonAcD neurons treated with KCl (depolarization), NaCl (control) and TTX (silencing) for 3 and 48 h. Mean ± SEM, three independent cultures, short-term plasticity: n (KCl) = 298 cells, n (NaCl) = 264 cells, n (TTX) = 306 cells, Chronic plasticity: n (KCl) = 174 cells, n (NaCl) = 184 cells, n (TTX) = 192 cells. Grey dot indicates value of individual cell. Orange and cyan triangle indicate mean of each independent culture. (C) Representative images of the AIS in AcD neurons upon induction of short-term (top row) and chronic (bottom row) plasticity. Neurons were treated with KCl for depolarization, NaCl as control, and TTX for silencing. Red arrowheads in upper row indicate the start and end of AIS. Red dashed line in bottom row indicates the start of the axon. Scale bar is 10 µm. (D) Measurement of AIS length and AIS distance in AcD neurons treated with KCl (depolarization), NaCl (control) and TTX (silencing) for 3 and 48 h. Mean ± SEM, Short-term plasticity: three independent cultures, n (KCl) = 48 cells, n (NaCl) = 40 cells, n (TTX) = 41 cells, Chronic plasticity: four independent cultures, n (KCl) = 82 cells, n (NaCl) = 72 cells, n (TTX) = 63 cells. Grey dot indicates value of individual cell. Orange and cyan triangle indicate mean of each independent culture. One-way ANOVA with Tukey’s multiple comparisons test, no significance (n.s.) P > 0.05, ***P < 0.001. More detailed statistical information see Data S1.

Short-term and chronic plasticity of the AIS in AcD neurons. (A) Representative images of the AIS in nonAcD neurons upon induction of short-term (top row) and chronic (bottom row) plasticity. Neurons were treated with KCl for depolarization, NaCl as control and TTX for silencing of activity. Red arrowheads in upper row indicate the start and end of AIS. Red dashed line in bottom row indicates the start of the axon. Scale bar is 10 µm. (B) Measurement of AIS length and AIS distance in nonAcD neurons treated with KCl (depolarization), NaCl (control) and TTX (silencing) for 3 and 48 h. Mean ± SEM, three independent cultures, short-term plasticity: n (KCl) = 298 cells, n (NaCl) = 264 cells, n (TTX) = 306 cells, Chronic plasticity: n (KCl) = 174 cells, n (NaCl) = 184 cells, n (TTX) = 192 cells. Grey dot indicates value of individual cell. Orange and cyan triangle indicate mean of each independent culture. (C) Representative images of the AIS in AcD neurons upon induction of short-term (top row) and chronic (bottom row) plasticity. Neurons were treated with KCl for depolarization, NaCl as control, and TTX for silencing. Red arrowheads in upper row indicate the start and end of AIS. Red dashed line in bottom row indicates the start of the axon. Scale bar is 10 µm. (D) Measurement of AIS length and AIS distance in AcD neurons treated with KCl (depolarization), NaCl (control) and TTX (silencing) for 3 and 48 h. Mean ± SEM, Short-term plasticity: three independent cultures, n (KCl) = 48 cells, n (NaCl) = 40 cells, n (TTX) = 41 cells, Chronic plasticity: four independent cultures, n (KCl) = 82 cells, n (NaCl) = 72 cells, n (TTX) = 63 cells. Grey dot indicates value of individual cell. Orange and cyan triangle indicate mean of each independent culture. One-way ANOVA with Tukey’s multiple comparisons test, no significance (n.s.) P > 0.05, ***P < 0.001. More detailed statistical information see Data S1.

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