Figure S2.
Trafficking of axonal and dendritic cargoes in nonAcD and AcD neurons. (A) Average number of pause of TfR vesicles travelling in the indicated regions of nonAcD and AcD neurons (regardless of directions). Mean ± SEM, five independent cultures, AIS (nonAcD) n = 25 cells, AIS (AcD) n = 23 cells, Stem D n = 23 cells, AcD n = 19 cells, Regular D n = 56 cells. (B) Total pausing duration (left) and time of each pause (right) of TfR vesicles travelling in the indicated regions of nonAcD and AcD neuron (regardless of directions). Mean ± SEM, five independent cultures, AIS (nonAcD) n = 25 cells, AIS (AcD) n = 23 cells, Stem D n = 23 cells, AcD n = 19 cells, Regular D n = 56 cells. (C–E) Velocity (C), run length (D) and run duration (E) of TfR vesicles running towards anterograde and retrograde directions in the AIS of nonAcD neuron, the AIS, stem dendrite (Stem D) and AcD of AcD neuron, and the regular dendrite (Regular D) of both nonAcD and AcD neuron. Mean ± SEM, five independent cultures, Anterograde: AIS (nonAcD): n = 21 cells, AIS (AcD) n = 15 cells, Stem D: n = 23 cells, AcD: n = 19 cells, Regular D: n = 54 cells, Retrograde: AIS (nonAcD) n = 23 cells, AIS (AcD) n = 23 cells, Stem D n = 20 cells, AcD n = 18 cells, Regular D n = 56 cells. (F) Percentage of EGFP-Rab3A vesicles change directions during anterograde transport (left) and the average number of direction changes (right). Mean ± SEM, seven independent cultures, AIS (nonAcD) n = 42 cells, AIS (AcD) n = 42 cells, Stem D n = 28 cells, Regular D n = 27 cells. (G–I) Average number of pause and pausing duration of EGFP-Rab3A vesicles travelling within the indicated region (regardless of direction). Average number of pauses per minute (G), average duration of each pause (H) and total pausing duration of EGFP-Rab3A vesicles during anterograde trafficking (I). Mean ± SEM, seven independent cultures, AIS (nonAcD) n = 42 cells, AIS (AcD) n = 42 cells, Stem D n = 28 cells, Regular D n = 27 cells. One-way ANOVA with Tukey’s multiple comparisons test, no significance (n.s.) P > 0.05, ****P < 0.0001.

Trafficking of axonal and dendritic cargoes in nonAcD and AcD neurons. (A) Average number of pause of TfR vesicles travelling in the indicated regions of nonAcD and AcD neurons (regardless of directions). Mean ± SEM, five independent cultures, AIS (nonAcD) n = 25 cells, AIS (AcD) n = 23 cells, Stem D n = 23 cells, AcD n = 19 cells, Regular D n = 56 cells. (B) Total pausing duration (left) and time of each pause (right) of TfR vesicles travelling in the indicated regions of nonAcD and AcD neuron (regardless of directions). Mean ± SEM, five independent cultures, AIS (nonAcD) n = 25 cells, AIS (AcD) n = 23 cells, Stem D n = 23 cells, AcD n = 19 cells, Regular D n = 56 cells. (C–E) Velocity (C), run length (D) and run duration (E) of TfR vesicles running towards anterograde and retrograde directions in the AIS of nonAcD neuron, the AIS, stem dendrite (Stem D) and AcD of AcD neuron, and the regular dendrite (Regular D) of both nonAcD and AcD neuron. Mean ± SEM, five independent cultures, Anterograde: AIS (nonAcD): n = 21 cells, AIS (AcD) n = 15 cells, Stem D: n = 23 cells, AcD: n = 19 cells, Regular D: n = 54 cells, Retrograde: AIS (nonAcD) n = 23 cells, AIS (AcD) n = 23 cells, Stem D n = 20 cells, AcD n = 18 cells, Regular D n = 56 cells. (F) Percentage of EGFP-Rab3A vesicles change directions during anterograde transport (left) and the average number of direction changes (right). Mean ± SEM, seven independent cultures, AIS (nonAcD) n = 42 cells, AIS (AcD) n = 42 cells, Stem D n = 28 cells, Regular D n = 27 cells. (G–I) Average number of pause and pausing duration of EGFP-Rab3A vesicles travelling within the indicated region (regardless of direction). Average number of pauses per minute (G), average duration of each pause (H) and total pausing duration of EGFP-Rab3A vesicles during anterograde trafficking (I). Mean ± SEM, seven independent cultures, AIS (nonAcD) n = 42 cells, AIS (AcD) n = 42 cells, Stem D n = 28 cells, Regular D n = 27 cells. One-way ANOVA with Tukey’s multiple comparisons test, no significance (n.s.) P > 0.05, ****P < 0.0001.

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