Scalability was provided by automatic segmentation. (A) 3D representation of a synaptosome slice visualized in an orthogonal view. (B) Probability map output by the neural network. The map is represented with a color gradient ranging from blue (0.9968) to red (1), indicating the likelihood of SV presence. (C) Segmentation after global threshold optimization. (D) The final representation of SVs segmentation post-processing. CryoVesNet segmented vesicles are depicted in light blue, combined with expert annotation in yellow (restricted within 250 nm of the active zone shown in red). Scale bar, 100 nm.