Multidimensional outlier detection. The scatter plot (left panel) represents vesicle features in the space defined by membrane intensity, radius, and thickness, with points colored according to the P value of their Mahalanobis distance, identifying potential outliers. Central panels: outliers are highlighted. Right panels: outliers have been either removed (top and middle row) or fixed by refinement(bottom row). In addition, the right panels show the final vesicle segmentation boundaries. Scale bars, 50 nm.