Adaptative local thresholding. (A) Virtual section through a probability map overlaid on the corresponding tomogram. The probability map is shown as colored contours ranging from 0.5 to 1 and logarithmically spaced (steps get finer and finer from 0.5 to 1). Red box size: 305 × 180 nm. (B) Probability map close-up corresponding to the red box in A. In the center, two SVs were initially detected as a single object. All the contours lower or equal to 0.8210 detect a single object. The contours equal to 0.9128 and above detect two separated objects.